Nanotechnology World Conference 2026

Abstract

Objective Bone defect remains a challenging clinical problem. Based on the theory of synergistic repair of bone immunity and bone regeneration, this study aims to integrate transcriptomics, proteomics, and phosphoproteomics to explore the intrinsic causal relationship and related molecular biological mechanisms of biomimetic micro-nanofiber materials in regulating the bone immune microenvironment during osteogenic differentiation.

Methods Biomimetic micro nanofiber materials were prepared using electrospinning technology. First, they were co cultured with macrophages, and the expression levels of macrophage M2 polarization markers were observed by flow cytometry, with further detection of the effects on polarization marker expression at both protein and gene levels. Subsequently, the supernatant of macrophage culture medium treated with biomimetic micro nanofiber materials was collected and co incubated with bone marrow mesenchymal stem cells BMSCs at different ratios. Transcriptomic, proteomic, and phosphoproteomic sequencing analyses were performed to jointly target the signaling pathway ranked first in the KEGG enrichment results, and rescue experiments were conducted for verification. Finally, biomimetic micro nanofiber materials were implanted into a rat critical sized calvarial defect model. Rat behavior and incision infection were observed, and material absorption was compared. New bone formation at the bone defect site was detected by Micro CT the repair of calvarial defect sites was observed by HE tissue sections the distribution of calcium nodules was detected by alizarin red staining the proportion of M2 type macrophage positivity was labeled by immunofluorescence the expression levels of M1 M2 markers were detected by flow cytometry and the expression levels of osteogenic differentiation related proteins ALP, Runx2, COL I, OCN, and OPN were detected by immunofluorescence. Rat BMSCs were sorted by flow cytometry, and the protein and phosphorylation levels of the signaling pathway were detected by Western Blot.